When choosing the correct HPLC column, you should consider the purpose of your separation. For example, if your aim is to separate two species, you need a column that has a high resolution. A column with a resolution of 1.5 or higher is the optimal choice. The length of the column should be based on the separation goal. Typically, a 15 cm column with a backpressure of 11 MPa is suitable.
When selecting a column, it is important to know how the columns behave under various conditions. Read the specification sheet carefully and follow the recommended operating conditions. Sometimes you may need to use your column outside its recommended flow rate. In this case, you should monitor its performance carefully to ensure that it is operating at optimum efficiency.
The type of column used in a particular application should also be considered. Some columns are not suitable for certain types of solvents. You may need a column that is suitable for a certain chromatography method. Some of these columns require multiple phases. For instance, an ideal HPLC system may require two or more different columns for different applications. Get helpful resources today!
High-quality HPLC columns should meet the highest standards. For example, a 10-um column may be too big for your needs. Choosing a smaller diameter is a better option. It will give you a greater capacity. For example, a smaller internal diameter will result in better packing. A larger internal diameter may limit the pump flow rate, which can reduce the efficiency of the instrument. Know more about HPLC Column Supplier today!
BIA Separations is a biotechnology company in Slovenia. In May 2008, it acquired a patent for a monolith column. The monolith column is a short disc that is encapsulated in a plastic housing. BIA Separations subsequently developed a full line of polymeric, ion-exchange, and affinity polymer monoliths. It also developed a large scale polymeric column called the Chromolith.
The company has a history of superior chemical products, but it's not particularly known for LC columns. However, it has sublicensed the technology to Phenomenex, a company known for its cutting-edge column technology. The Onyx line was first introduced in January 2005. The company has maintained strict quality controls and passes strict criteria to ensure quality.
An HPLC column is expensive and requires proper maintenance. It must be optimized to maximize its efficiency. For example, it should be capable of extra-column band broadening. In addition, troubleshooting situations may involve the column, sample, mobile phases, and instrumentation. For size exclusion chromatography, the column should be cleaned properly.
Certain SEC applications require surfactants to be used. This can affect the retention time and increase the resolution. For this reason, a dedicated SEC column is better suited for these types of tests. Read more about chromatography at https://en.wikipedia.org/wiki/High-performance_liquid_chromatography.