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    Choosing an HPLC Column Supplier

    HPLC columns are used in a wide variety of applications including analytical, forensic, and clinical testing labs. The pharmaceutical industry is the biggest driver of technological advancements in this area. High-throughput monolithic column technologies are expected to have the greatest economic impact. In order to increase throughput, LC columns have been improved in many ways.

     

    HPLC columns typically consist of high-purity particulate silica compressed into a stainless steel tubing. The smaller the particle size, the lower the diffusion distance and, thus, the higher the selectivity. As a result, decreasing particle size also increases backpressure. This is because pressure is inversely proportional to particle size. The decrease in particle size reduces interstitial voids and allows a compound to push through the column faster.

     

    HPLC column longevity is an important factor for chromatographers. Some chromatographers track column injections in their logbooks. However, many do not. In fact, many will simply install a HPLC column and leave it in their instrument until they notice a significant amount of tailing or resolution loss. Some will even document the time spent in a logbook. Read this article for more details!

     

    For optimal separation, the optimum column length is 15 cm. The longer column length provides more resolution, but this should be based on the separation objectives. In general, a 15-cm column operating at 11 Mpa backpressure is well within the operating range. There are several considerations in choosing the best column length for your application. Be sure to read more today!

     

    In addition to the retention time, there are other important factors for HPLC analysis. While the lifespan of the columns is increasing, users still need seven new columns per instrument per year. This may be influenced by increased sample volumes or the procurement of improved columns. A few factors may also influence the decision to buy a new HPLC column.

     

    One important aspect to consider when choosing the right HPLC column is the type of column you want. Some columns have an inert matrix. A fast desalting SEC column should have a high pore volume and large pore size distribution. A dedicated SEC column is preferred for applications where additives are used. Ensure that the column you choose has an OCS sheet that specifies if it can handle the additives you are using.

     

    The next consideration is the separation parameters. You may need to modify your sample concentrations and method. The method will need further development if you want to achieve the best separation. For example, if you want to determine the concentration of a specific protein, you may use a monoclonal antibody as an internal standard. If not, you can also use NIST or USP standards for the same purpose.

     

    Another factor to consider is the manufacturer of the HPLC column. While a high-quality column will be expensive, a high-quality column will provide optimal separation performance. A good column supplier will also provide technical support and maintenance. Get more facts about chromatography at https://www.britannica.com/summary/chromatography.

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